All the genotypes of mice and cells were verified at genomic DNA and cDNA level, respectively

ted animals always targeted a single location. As expected, the treated antennal lobes showed minimal NP glial cell body migration along glomerular boundaries, but the labeled axon terminal branches always clustered adjacent to the primary neurite tract of the medial group of AL neurons, as they did in controls. It therefore appears Glial FGFRs in Glia-Neuron Signaling development and organization of mesodermal structures including heart and somatic muscles in the embryo, and Breathless, with 5 Ig domains, important in development of the tracheal system of the embryo. Heartless is expressed in longitudinal glial cells and both FGFRs are important in embryonic CNS development. The only other evidence for involvement in the post-embryonic CNS was reported in a brief study of 3rd instar Drosophila in which Heartless, but not Breathless, mRNA was found in eye-antenna imaginal discs. The current work in Manduca focuses on the developing adult, rather than embryonic or larval stages, however, making comparison with the Drosophila studies difficult. The important point here is that, in order R-115777 metamorphic Glial FGFRs in Glia-Neuron Signaling adult development in Manduca, the FGFR is expressed by CNS and peripheral glia, and not by tracheae. High magnification imaging of antennal-lobe and antennal-nerve glia revealed the presence of FGFRs on glial processes but also closely associated with nuclear DNA. DNA labeled with Syto 13 appears to be concentrated into “chromosome territories” associated with intranuclear pFGFRs. We are not aware of other descriptions of nuclear localization of FGFRs in invertebrates, but this phenomenon has been described in cultured fibroblasts and in human astrocytes and glioma cells, where nuclear localization appears to be correlated with transcriptional regulation and subsequent glial-cell proliferation. Further work is needed to determine whether or not nuclear localization of “25849133 FGFRs can be connected to specific cellular functions in invertebrates. Heartless expression also has been reported in embryonic Drosophila neurons grown in culture and in vivo. We likewise saw evidence of FGFRs in the AL neurons, but only in their cell bodies, not in their dendrites or axons. There is evidence that FGFRs can be imported directly from endoplasmic reticulum to the nucleus without ever being expressed on the plasma membrane. This latter phenomenon, termed “integrative nuclear FGFR signaling”may be relevant to our observation that FGFR labeling in the AL neurons is limited to their cell bodies, and might help explain why AL neuron cell bodies in PD173074-treated animals continue to label for activated FGFRs. In this scenario, activation of signaling pathways within AL neurons would lead to direct translocation of FGFRs from the endoplasmic reticulum to the nucleus in order to modulate ” gene transcription. The nature of the role of FGFRs in AL neurons remains unanswered. Heparan sulfate proteoglycans have been described as essential co-receptors for FGFs. As was the case for pFGFRs, we found HSPGs expressed in glial cells and AL neurons. Additionally, we found HSPGs both on cell processes and in nuclei. This, too, is in agreement with published accounts that HSPG localization can vary. We have shown previously that ORNs express EGFRs and find here that these EGFRs are activated normally following treatment with PD173074. If ORN EGFR activation had been blocked, ORN axons would have stalled in the sorting zone, making it thicker than norma