Vary as a function of rainfall, NDVI and temperatures [29,30]. ?Human related

Vary as a function of rainfall, NDVI and temperatures [29,30]. ?Human related factors: habitat, gender, profession, contact with ruminant and ruminant products [31]. Values of covariates were computed at the commune level (except for human behaviors which were at the individual level).PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.July 14,4 /Rift Valley Fever Risk Factors in MadagascarCattle density. For each of the 1,578 communes considered, cattle density was estimated using the new global PP58 manufacturer distribution maps for cattle produced by the Food and Agriculture Organization of the United Nations (FAO; http://www.fao.org/Ag/againfo/resources/en/glw/GLW_ dens.html; [32]). Water bodies and landscape classes. A landscape map of Madagascar was obtained from Globcover project [33]. The GlobCover 2009 landscape product is a 300-m global landscape map produced from an automated classification of Medium Resolution Imaging Spectrometer (MERIS) time series. The global landscape map included 22 landscape classes defined with the United Nations (UN) Land Cover Classification System (LCCS). Among these 22 classes, we identified 5 relevant LCCS categories: “Cultivated Terrestrial Areas and Managed Lands” (so-called Crops), “Woody/ Trees”, “Shrubs”, “Herbaceous”, “Artificial Surfaces (so-called Urbanization)”. To reflect the availability of potential breeding habitats of RVF vectors in Madagascar such as artificial, irrigated, permanent and temporary water bodies, we needed to combine different data sources extracted from several GIS databases. The first one described inland permanent water point, such as lake, and was available from DIVA-GIS (http://www.diva-gis.org/). Marshland data representing temporary water bodies were obtained from Geographical Information Systems at the Royal Botanic Gardens, Kew [34]. Wetland locations representing temporary water bodies were extracted from the International Panel on Climate Change (IPCC; [35]). Irrigated area locations came from Global Map of Irrigation Areas (GMIA) from AQUASTAT-FAO [36]. Climatic variables: Precipitation, temperatures and NDVI. To depict the climatic conditions at each commune of Madagascar, day and night Land Surface Temperature (LST) and NDVI were retrieved from Moderate Resolution Imaging Spectroradiometer (MODIS; http:// iridl.ldeo.Saroglitazar Magnesium dose columbia.edu/). For the period 2001 to 2010, day and night LST were extracted from MODIS data produced every 8 days at 1 km spatial resolution (MODIS MOD11A2 product: Land Surface Temperature and Emissivity). For the same period, NDVI data were obtained from MODIS data produced every 16 days at 250 m spatial resolution (MODIS MOD13A1: Vegetation Indices). Rainfall data were retrieved from the Tropical Rainfall Measuring Mission (TRMM; http://pmm.nasa.gov/trmm/mission-end). These data were produced at 25 km spatial resolution. Finally, for each commune and the same period, we computed the annual mean of day and night LST, NDVI and precipitation. Seasonality of NDVI and precipitation was also considered by computing the difference between the cumulated value over 3 months of the rainy season (November, December and January) and the cumulated value over 3 months of the dry season (June, July and August). Human related factors. Human density was computed for each commune using data generated from Landscan 2007 Global Population Grid from Oak Ridge National Laboratory the US Department of Defense (OCHA, 2007). Based on our field knowledge, the communes w.Vary as a function of rainfall, NDVI and temperatures [29,30]. ?Human related factors: habitat, gender, profession, contact with ruminant and ruminant products [31]. Values of covariates were computed at the commune level (except for human behaviors which were at the individual level).PLOS Neglected Tropical Diseases | DOI:10.1371/journal.pntd.July 14,4 /Rift Valley Fever Risk Factors in MadagascarCattle density. For each of the 1,578 communes considered, cattle density was estimated using the new global distribution maps for cattle produced by the Food and Agriculture Organization of the United Nations (FAO; http://www.fao.org/Ag/againfo/resources/en/glw/GLW_ dens.html; [32]). Water bodies and landscape classes. A landscape map of Madagascar was obtained from Globcover project [33]. The GlobCover 2009 landscape product is a 300-m global landscape map produced from an automated classification of Medium Resolution Imaging Spectrometer (MERIS) time series. The global landscape map included 22 landscape classes defined with the United Nations (UN) Land Cover Classification System (LCCS). Among these 22 classes, we identified 5 relevant LCCS categories: “Cultivated Terrestrial Areas and Managed Lands” (so-called Crops), “Woody/ Trees”, “Shrubs”, “Herbaceous”, “Artificial Surfaces (so-called Urbanization)”. To reflect the availability of potential breeding habitats of RVF vectors in Madagascar such as artificial, irrigated, permanent and temporary water bodies, we needed to combine different data sources extracted from several GIS databases. The first one described inland permanent water point, such as lake, and was available from DIVA-GIS (http://www.diva-gis.org/). Marshland data representing temporary water bodies were obtained from Geographical Information Systems at the Royal Botanic Gardens, Kew [34]. Wetland locations representing temporary water bodies were extracted from the International Panel on Climate Change (IPCC; [35]). Irrigated area locations came from Global Map of Irrigation Areas (GMIA) from AQUASTAT-FAO [36]. Climatic variables: Precipitation, temperatures and NDVI. To depict the climatic conditions at each commune of Madagascar, day and night Land Surface Temperature (LST) and NDVI were retrieved from Moderate Resolution Imaging Spectroradiometer (MODIS; http:// iridl.ldeo.columbia.edu/). For the period 2001 to 2010, day and night LST were extracted from MODIS data produced every 8 days at 1 km spatial resolution (MODIS MOD11A2 product: Land Surface Temperature and Emissivity). For the same period, NDVI data were obtained from MODIS data produced every 16 days at 250 m spatial resolution (MODIS MOD13A1: Vegetation Indices). Rainfall data were retrieved from the Tropical Rainfall Measuring Mission (TRMM; http://pmm.nasa.gov/trmm/mission-end). These data were produced at 25 km spatial resolution. Finally, for each commune and the same period, we computed the annual mean of day and night LST, NDVI and precipitation. Seasonality of NDVI and precipitation was also considered by computing the difference between the cumulated value over 3 months of the rainy season (November, December and January) and the cumulated value over 3 months of the dry season (June, July and August). Human related factors. Human density was computed for each commune using data generated from Landscan 2007 Global Population Grid from Oak Ridge National Laboratory the US Department of Defense (OCHA, 2007). Based on our field knowledge, the communes w.