Ansferase theta 1 (GSTT1), and caspase three (CASP3). p 0.05, p

Ansferase theta 1 (GSTT1), and caspase three (CASP3). p 0.05, p 0.01.4.4. Discussion Discussion In this study, the transcriptome the edible red sea urchin (L. albus) was sequenced In this study, the transcriptome ofof the edible red sea urchin (L. albus) was sequenced and annotated by using NGS technology. That is the first report on the RNA sequencand annotated by using NGS technology. That is the initial report on the RNA sequencing, transcriptome assembly, and functional annotation from juvenile L. albus according to on ing, transcriptome assembly, and functional annotation from juvenile L. albus basedthree different tissues: Bentiromide Biological Activity intestines, gonads, and coelomocytes. transcriptome contains 3 unique tissues: intestines, gonads, and coelomocytes. This transcriptome Dicyclanil Epigenetics consists of 185,239 transcripts with similar featuresexisting transcriptomes of otherother sea urchins. to current transcriptomes of sea urchins. For 185,239 transcripts with related options to For instance, the GC content material of L. albus includes a similar value in relation for the sea the sea instance, the GC content material of L. albus (38.8 )(38.eight ) has a related value in relation tourchin urchin transcriptomes of Evechinus chloroticus [32], Sterechinus neumayeri (38.six ) [33], transcriptomes of Evechinus chloroticus (39.0 ) (39.0 ) [32], Sterechinus neumayeri (38.six ) [33], Strongylocentrotus intermedius (39.6 ) Mesocentrotus nudus (39.9 ) [35]. However, Strongylocentrotus intermedius (39.six ) [34], and [34], and Mesocentrotus nudus (39.9 ) [35]. Nevertheless, our results exhibit a slightly decrease than lower reported for the testis transcripour outcomes exhibit a GC content GC content slightly values than values reported for the testis transcriptome of L. albus This smaller This smaller difference could for the tissue to the tissue tome of L. albus (40.four ) [6]. (40.4 ) [6].distinction may be attributedbe attributed utilized in the study (mature gonad), as well because the sequencingthe sequencing technology (Roche 454 GSused in the study (mature gonad), too as technology (Roche 454 GS-FLX Titanium). This group obtained 1062,716 raw reads having a imply length of imply length of 309.eight bp, FLX Titanium). This group obtained 1062,716 raw reads with a 309.8 bp, producing a reference transcriptometranscriptome of 42,530 transcripts with an N50 of 645 bp [6], inour producing a reference of 42,530 transcripts with an N50 of 645 bp [6], in contrast to constudy, to our study, which presents 91,119,300 paired finish mean length of 250 length of 250 bp, trast which presents 91,119,300 paired finish reads having a reads using a mean bp, producing a creating a reference transcriptome of 185,239with an N50 of 1769N50 of 1769 bp. reference transcriptome of 185,239 transcripts transcripts with an bp. AA detailed evaluation tissue expression reveal that that 26,864 transcripts are differendetailed evaluation of of tissue expression reveal 26,864 transcripts are differentially expressed among the three tissues. Coelomocytes are the cells responsible for immunity in tially expressed among the three tissues. Coelomocytes will be the cells responsible for imsea urchins, seawhich 50 to 70 are motile cells using a high energyarequirement and are munity in of urchins, of which 50 to 70 are motile cells with higher energy requireconsidered equivalent to human macrophages, which are cellular immune cellular compoment and are deemed equivalent to human macrophages, which are program immune nents with high catabolic activity and are a part of the innate i.