Employed by the temperature and ethanol concentration within the extraction buffer. Accordingly, we have been

Employed by the temperature and ethanol concentration within the extraction buffer. Accordingly, we have been in a position to define an optimal protocol depending on the extraction of red chicory powder at 4 C for 30 min applying 50 ethanol containing 2 tartaric acid as the solvent, matching the efficiency of your gold-standard protocol depending on methanol acidified with 2 HCl beneath precisely the same circumstances (no considerable distinction observed inside a t-test, p 0.05). We characterized the extracts by evaluating their stability more than time when stored as pure extracts, three-fold concentrates, or lyophilized powders at two distinct tem-Molecules 2021, 26,14 ofperatures (4 and 23 C). We located that the lyophilization of aqueous extracts (extraction buffer = 2 tartaric acid in water with no ethanol) followed by storage at 4 C preserved the anthocyanin contents for six months, PF-06454589 Autophagy maximum yields of 65.3 mg/100 g LFW by extraction with 50 methanol containing 4 formic acid at area temperature, whereas Migliorini et al. [9] accomplished maximum yields of 73.53 0.13 mg/100 g LFW by extraction with water acidified with acetic acid (pH two.5 at 62.four C). Red chicory leaves have previously been shown to accumulate different anthocyanins, especially cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O-(6-malonyl)glucoside, cyanidin-3-O-rutinoside, cyanidin-3,5-di-O-(6-O-malonyl)-glucoside, cyanidin3-O-(-O-acetyl)-glucoside, and cyanidin-3-O-gluc.