Kina1, Joel Z. Nordin3, Samir ELAndaloussi3,two and Matthew J. Wood2 University of Oxford, Oxford, UK;

Kina1, Joel Z. Nordin3, Samir ELAndaloussi3,two and Matthew J. Wood2 University of Oxford, Oxford, UK; 2Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK; 3Department of Laboratory Medicine, Karolinska Instiutet, Stockholm, SwedenPS02.Enzymatic exosomes with GPI-anchored hyaluronidase for enhanced tumour penetration and anti-tumour efficacy Yeon-Sun Hong1, Yoosoo Yang2 and In-San Kim1 KU-KIST Graduate School of Converging Science and Technologies, Korea University; 2Korea Institute of Science and TechnologyPlease see OPT01.PS02.Efficient delivery of glucocerebrosidase lysosomal enzyme by means of EXPLOR technology for treatment of Gaucher disease Hojun Choi1, Kyungsun Choi1, Nambin Choi1, Seung Wook Choi2 and Chulhee ChoiIntroduction: Exosomes are readily taken up by several cell sorts, in what appears to be an energy-dependent, directed procedure. Moreover exosomes have already been described to transport a range of Small Ubiquitin-Like Modifier 4 Proteins site bioactive molecules including proteins, lipids and nucleic acids. With each other with their possible non-immunogenic properties, exosomes facilitate a new paradigm within the delivery of therapeutic agents. Even so, owing to their biogenesis mechanisms, exosomes usually are not readily enriched with targeted soluble proteins without anchoring for the exosomal membrane or incurring bulky fusion modifications. Right here we demonstrate that utilising an exosomal co-localisation signal, in addition to a self-cleaving protein we are capable to enrich for precise soluble proteins within the exosomal lumen. Strategies: DNA constructs have been generated by introducing the selfcleaving intein, IC-TM, downstream of your CD63 ORF. Following the intein region a reporter ORF of interest was inserted. Exosomes containing the self-cleaving constructs have been generated in HEK293t cells and characterised by NTA and western blotting. Finally the reporter enriched exosomes have been co-incubated with recipient cell lines and analysed by confocal microscopy or suitable readout assay. Benefits: Here we show that exosomes are successfully enriched with the reporter protein, independent of your co-localisation signal-intein fusion. NTA and western blot analysis of the vesicles suggests tiny to no variation from their wild type counterparts. Lastly, exosomes enriched with the reporter proteins are readily taken up by recipient cells, and display proof of cargo protein assimilation Conclusion: Right here we describe a novel technique of enriching exosomes having a soluble protein independent of remnant co-localisation fusions. These enriched exosome have been demonstrated to deliver their cargo to recipient cells. We envisage this approach applicable to both simple and therapeutic biology alike.KAIST, Seoul, Republic of Korea; 2Cellex Life Sciences, IncSaturday, May 20,PS02.Delivery of membrane-bound CD39/CD73 by extracellular vesicles (EVs) for treatment of inflammatory disease Susanne A. Snoek1, Niels Broekstra1, Jan van Ittersum1, Jeroen de Vrij2, Edwin van der Pol3, Rienk Nieuwland4, Lisa G.M. van Baarsen5, Paul P. Tak1, Margriet Vervoordeldonk1 and Jonathan FinnArthrogen BV; 2Department of Frizzled-4 Proteins Formulation Neurology, Erasmus Health-related Center; 3Biomedical Engineering Physics and Vesicles Observation Center, Academic Health-related Center; 4Clinical Chemistry department, Academisch Medisch Centrum; 5 Clinical Immunology and Rheumatology, Academic Healthcare CenterIntroduction: Our recent information demonstrated that the balance amongst pro-inflammatory extracellular ATP and anti-inflammatory adenosine is skewed within the synovial com.