nose synthase TUBA; tubulin alpha RFA1, RPA1, rpa; replication element A1 UXS1, uxs; UDP-glucuronate decarboxylase

nose synthase TUBA; tubulin alpha RFA1, RPA1, rpa; replication element A1 UXS1, uxs; UDP-glucuronate decarboxylase CCT3, TRIC5; T-complex protein 1 subunit gamma CYP90A1, CPD; cytochrome P450 loved ones 90 subfamily A1 CPVL; vitellogenic carboxypeptidase-like protein NR; nitrate reductase (NAD(P)H) UBE2A, UBC2, RAD6A; ubiquitin-conjugating enzyme E2 A ALKBH5; mRNA N6-methyladenine demethylase sufS; cysteine desulfurase / selenocysteine lyase PRR5; pseudo-response regulator 5 NFX1; transcriptional repressor NF-X1 HNRNPA1_3; heterogeneous nuclear ribonucleoprotein A1/A3 Pathway termsBhattarai et al. BMC Plant Biology(2021) 21:Page 8 ofTable two Variety of IL-12 Inhibitor web differentially expressed genes (DEGs) and corresponding pathways in leaf and root of salt tolerant `Halo’ and salt intolerant `Vernal’ alfalfa cultivars at 0, three and 27 h of salt tension (Continued)Pathway ID K12891 K13946 K13963 K14315 K14404 K14488 K14504 K14568 K14842 K15281 K15378 K15397 K15747 K16298 K17525 K17592 K17679 K18270 K18857 K20471 K20628 K20726 K21797 K23050 K23570 The amount of differentially expressed genes HL0vsVL0 HL3vsVL3 HL27vsVL27 HR0vsVR0 HR3vsVR3 HR27vsVR27 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 1 0 0 1 1 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1 0 1 0 0 0 1 0 0 0 0 1 1 1 0 0 0 0 1 0 1 1 1 1 0 1 1 1 0 0 0 1 1 1 0 0 0 1 0 0 1 1 1 0 0 1 1 1 1 0 1 1 1 1 1 0 1 0 0 1 0 0 1 0 1 0 0 1 0 1 1 0 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 SFRS2; splicing issue, arginine/serine-rich 2 AUX1, LAX; auxin influx carrier (AUX1 LAX loved ones) SERPINB; serpin B NDC1, TMEM48; nucleoporin NDC1 CPSF4, YTH1; cleavage and polyadenylation specificity element subunit 4 SAUR; SAUR family protein TCH4; xyloglucan:xyloglucosyl transferase TCH4 EMG1, NEP1; rRNA little subunit pseudouridine methyltransferase Nep1 NSA2; ribosome biogenesis protein NSA2 SLC35D; solute carrier family 35 SLC45A1_2_4; solute carrier family 45, member 1/2/4 KCS; 3-ketoacyl-CoA synthase LUT5, CYP97A3; beta-ring hydroxylase SCPL-IV; serine carboxypeptidase-like clade IV CHID1; chitinase domain-containing protein 1 SACS; sacsin MSS116; ATP-dependent RNA helicase MSS116, mitochondrial RAB3GAP1; Rab3 GTPase-activating protein catalytic subunit ADH1; alcohol dehydrogenase class-P COPD, ARCN1, RET2; coatomer subunit delta exlX; expansin TMEM222; transmembrane protein 222 SAC1, SACM1L; phosphatidylinositol 4-phosphatase PCBER1; phenylcoumaran benzylic ether reductase EMC10; ER membrane protein complex subunit ten Pathway termsHL0 Halo leaf handle, VL0 Vernal leaf handle, HL3 Halo leaf soon after three h of salt pressure, VL3 Vernal leaf after 3 h of salt tension, HL27 Halo leaf after 27 h of salt anxiety, VL27 Vernal leaf just after 27 h of salt pressure, HR0 Halo root manage, VR0 Vernal root control, HR3 Halo root after 3 h of salt stress, VR3 Vernal root after 3 h of salt anxiety, HR27 Halo root just after 27 h of salt tension, VR27 Vernal root after 27 h of salt stressconsistently expressed beneath short-term and long-term salt tension inside the salt tolerant cultivar. Fourth, this study identified 74,705 SNPs which are important marker for future alfalfa breeding for salt tolerance. Fifth, we found `Halo’ beneath salt strain maintained 5 5 times more DEGs within the root than `Vernal’. Lastly, this study located seven candidate genes for salt tolerance (MS.gene32989, MS.gene065734, MS.gene24746, MS.gene81767, MS.gene044457, MS.gene049840, and MS.gene46459) with unknown functions, suggesting a require for additional HSP90 Inhibitor Biological Activity analysis to understand their part in salt