Product Name :
RS-1, CRISPR Editing Enhancer
Description:
RS-1 was previously characterized as a RAD51-Stimulatory compound and now found to be an HDR enhancer to enhance CRISPR/Cas9- and TALEN-mediated knock-in efficiency. It increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects. RS-1 was applied for animal production and have achieved multifold improvement on the knock-in rates as well. RS-1 can stimulate the human homologous recombination protein RAD51in various conditions. In PC3 prostate cancer cells, RS-1–induced lethality was accompanied by the formation of microscopically visible RAD51 nuclear protein foci occurring in the absence of any DNA-damaging treatment. Treatment with RS-1 promoted significant antitumor responses in a mouse model.
CAS:
312756-74-4
Molecular Weight:
524.23
Formula:
C20H16Br2N2O3S
Chemical Name:
3-(N-benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide
Smiles :
O=C(NC1C=CC(Br)=CC=1)C1C=C(C(Br)=CC=1)S(=O)(=O)NCC1C=CC=CC=1
InChiKey:
SWKAVEUTKGKHSR-UHFFFAOYSA-N
InChi :
InChI=1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
RS-1 was previously characterized as a RAD51-Stimulatory compound and now found to be an HDR enhancer to enhance CRISPR/Cas9- and TALEN-mediated knock-in efficiency. It increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects.{{Loperamide} web|{Loperamide} GPCR/G Protein|{Loperamide} Protocol|{Loperamide} Purity|{Loperamide} custom synthesis|{Loperamide} Epigenetic Reader Domain} RS-1 was applied for animal production and have achieved multifold improvement on the knock-in rates as well. RS-1 can stimulate the human homologous recombination protein RAD51in various conditions. In PC3 prostate cancer cells, RS-1–induced lethality was accompanied by the formation of microscopically visible RAD51 nuclear protein foci occurring in the absence of any DNA-damaging treatment. Treatment with RS-1 promoted significant antitumor responses in a mouse model.|Product information|CAS Number: 312756-74-4|Molecular Weight: 524.23|Formula: C20H16Br2N2O3S|Chemical Name: 3-(N-benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide|Smiles: O=C(NC1C=CC(Br)=CC=1)C1C=C(C(Br)=CC=1)S(=O)(=O)NCC1C=CC=CC=1|InChiKey: SWKAVEUTKGKHSR-UHFFFAOYSA-N|InChi: InChI=1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO up to 100 mM|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.{{Acebilustat} medchemexpress|{Acebilustat} Metabolic Enzyme/Protease|{Acebilustat} Purity & Documentation|{Acebilustat} In stock|{Acebilustat} supplier|{Acebilustat} Cancer} |Shelf Life: ≥12 months if stored properly.PMID:24580853 |Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined.|HS Tariff Code: 382200|How to use|In Vitro:|RS-1 was used at 15 µM final concentration in TALEN- or Cas9-mediated knock-in experiments.|In Vivo:|RS-1 was used at 7.5 μM for the in vivo knock-in animal production. RS-1 can be dosed to animal by IP injection at 110 mg/Kg once per day to get antitumor responses.|References:|Jayathilaka K, et al. A chemical compound that stimulates the human homologous recombination protein RAD51. (2008) Proc Natl Acad Sci USA. 105(41):15848-53.Mason JM, et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. (2014) Cancer Res. 74(13):3546-55.Song J, et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. (2016) Nat Commun. 7:10548.Products are for research use only. Not for human use.|Documents||
