Product Name :
Streptavidin(iFluor488)
Applications:
ELISA,WB,Flow Cyt
Reactivity :
Biotin
Conjugate:
iFluor488
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Streptavidin(iFluor488) is coupled steptavidin with iFluor488. Streptavidin(iFluor488) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems. | Conjugate: iFluor488(Ex: 495nm, Em: 519nm), 2 moles iFluor488 per mole streptavidin | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, protect from light | Background:Streptavidin is a 53 kDa protein isolated from Streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and contains 4 subunits. Each subunit can bind one molecule of biotin. Streptavidin has strong binding properties to avidin (Kd=10-15). Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins.{{78110-38-0} web|{78110-38-0} Protocol|{78110-38-0} In Vivo|{78110-38-0} manufacturer}
Description2 :
Streptavidin(iFluor488) is coupled steptavidin with iFluor488. Streptavidin(iFluor488) is highly sensitive to binds biotinylated proteins in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry systems.
Immunogen:
Host :
Isotype:
Purity :
Buffer :
Storage :
Store at –20 °C, protect from light
Function:
WB: 1:10,000-1:50,000 ELISA: 1:10,000-1:50,000Flow Cyt: 1:200-1:2000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc.{{1190264-60-8} site|{1190264-60-8} Technical Information|{1190264-60-8} References|{1190264-60-8} manufacturer} The actual dilution used must be determined empirically.PMID:29261986
