Product Name :
Anti-HA tag, AlpSdAbs® VHH(Biotin)
Applications:
ELISA,Purification,BLI,SPR
Reactivity :
HA tag
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-HA tag, AlpSdAbs® VHH(Biotin) is designed for detecting HA tag fusion proteins. Anti-HA tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to HA tag coupled to Biotin, and Anti-HA tag, AlpSdAbs® VHH(Biotin) detects the HA tag selectively, no reactivity with other proteins. | Immunogen: HA tag fused KLH | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: Biotin | Specificity: HA tag(YPYDVPDYA) | Cross-Reactivity: Highly selective for HA tag sequencePurity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles) | Background:The HA tag is widely used for detecting, manipulating or purifying proteins.{{2775241-92-2} site|{2775241-92-2} Purity & Documentation|{2775241-92-2} References|{2775241-92-2} custom synthesis} This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.{{152121-30-7} web|{152121-30-7} Technical Information|{152121-30-7} In Vitro|{152121-30-7} manufacturer} Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(
Description2 :
Anti-HA tag, AlpSdAbs® VHH(Biotin) is designed for detecting HA tag fusion proteins. Anti-HA tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to HA tag coupled to Biotin, and Anti-HA tag, AlpSdAbs® VHH(Biotin) detects the HA tag selectively, no reactivity with other proteins.PMID:25905422
Immunogen:
HA tag fused KLH
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Store at –20 °C(Avoid freeze / thaw cycles)
Function:
WB: 1:5,000-1:20000 ELISA: 1:5,000-1:20000IP: 1-2ug/sampleBLI (biolayer interferometry)SPR (surface plasmon resonance)Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
