Product Name :
pCANTAB5E
Applications:
phage display system
Reactivity :
Conjugate:
Advantages :
Description:
This system is used for antibody gene expression and preparation of recombinant antibody. The host strain TG1 was replicated and expressed by plasmid vector pCANTAB5e, and was cultured on 2 *YT medium at 37 C. The vector pCANTAB5e is used to construct recombinant scFv. It has ampicillin resistance and the size is 4522 bp. The auxiliary phage M13K07 is used to rescue the phage particles. It is Kana-resistant and can be replicated by the auxiliary phage and expressed on the phage surface in the form of fusion. There is a sequence encoding Tag tail peptide (E-Tag) behind the scFv gene. There is an amber termination codon behind the tail peptide. It is located between the scFv gene and the cpIII gene. In the inhibitory bacteria TG1, only 20% of the amber codon is effective, so it can be read through the protein translation process to form scFv-cp. In non-inhibitory strains, such as HB2151, the terminator is recognized, and the scFv gene terminates before the cpIII gene in the translation process, forming an independent antibody protein that remains in the cell membrane gap, and leaks into the culture medium after a long period of culture to form soluble expression.SummaryFunction E.coli Cloning plasmidPromoter: Lac/lac promoterPlasmid size: 5216bpPlasmid tagging: N-g3 signal, C-E tagProkaryotic resistance: ampicillin AmpClone strain: Escherichia coli DH5αCulture conditions: 37 C, aerobic, LBVector sequence 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacgt gaaaaaatta 2281 ttattcgcaa ttcctttagt tgttcctttc tatgcggccc agccggccat ggcccaggtg 2341 aagctgcagc agtcaggacc tggcctggtg gcgccctcac agagcctgtc catcacatgc 2401 accgtctcag ggttttcatt aaccagctat ggtgtacact gggttcgcca gcctccagga 2461 aagggtctgg agtggctggg agtaatatgg gctggtggaa gcacaaacta taattcagct 2521 ctcaaatcca gactgaacat cagcaaggac aactccaaga gccaagtttt cttaaaaatg 2581 aacagtctcc aaactgatga cacagccatg tactactgtg ccagaaactg gggcagctac 2641 tggtacttcg atgtctgggg ccaaggccac ggtcaccgtc tcctcagtgg aggcggttca 2701 ggcggaggtg gcggaggtgg ctctggcggt ggcggatcgg acattgagct cacccagtct 2761 ccagcaatca tgtctgcatc tccaggggaa aaggtcacca tgacctgcag ggccagctca 2821 agtataagtt ccagttactt gcactggtac cagcagaagt caggcgcttc ccccaaaccc 2881 ttgattcata ggacatccaa cctggcttct ggagtcccag ctcgcttcag tggcagtggg 2941 tctgggacct cttactctct cacaatcagc agcgtggagg ctgaagatga tgcaacttat 3001 tactgccagc agtggagtgg ttacccattc acgttcggtg ctgggaccaa gctcgagatc 3061 aaacgggcgg ccgcaggtgc gccggtgccg tatccggatc cgctggaacc gcgtgccgca 3121 tagactgttg aaagttgttt agcaaaacct catacagaaa attcatttac taacgtctgg 3181 aaagacgaca aaactttaga tcgttacgct aactatgagg gctgtctgtg gaatgctaca 3241 ggcgttgtgg tttgtactgg tgacgaaact cagtgttacg gtacatgggt tcctattggg 3301 cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga gggtggcggt 3361 tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc gggctatact 3421 tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc cgctaatcct 3481 aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa taataggttc 3541 cgaaataggc agggtgcatt aactgtttat acgggcactg ttactcaagg cactgacccc 3601 gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga cgcttactgg 3661 aacggtaaat tcagagactg cgctttccat tctggcttta atgaggatcc attcgtttgt 3721 gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg cggcggctct 3781 ggtggtggtt ctggtggcgg ctctgagggt ggcggctctg agggtggcgg ttctgagggt 3841 ggcggctctg agggtggcgg ttccggtggc ggctccggtt ccggtgattt tgattatgaa 3901 aaaatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa cgcgctacag 3961 tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc tatcgatggt 4021 ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga ttttgctggc 4081 tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat gaataatttc 4141 cgtcaatatt taccttcttt gcctcagtcg gttgaatgtc gcccttatgt ctttggcgct 4201 ggtaaaccat atgaattttc tattgattgt gacaaaataa acttattccg tggtgtcttt 4261 gcgtttcttt tatatgttgc cacctttatg tatgtatttt cgacgtttgc taacatactg 4321 cgtaataagg agtcttaata agaattcact ggccgtcgtt ttacaacgtc gtgactggga 4381 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 4441 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 4501 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata 4561 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 4621 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat 4681 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 4741 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa 4801 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 4861 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 4921 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 4981 acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg acgggtgcag 5041 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 5101 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 5161 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga//
Description2 :
Immunogen:
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Purity :
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