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To investigate the result of this pathologic bladder microenvironment on SKP differentiation, to additional especially take a look at how the bladder pressure-induced microenvironment regulates the destiny perseverance of SKPs, we employed a blend of ex vivo organ society

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Image J was applied to quantify gel region right after contraction.SKPs were being plated...
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The ultimate final results had been exhibited as a pseudocolor photon rely picture superimposed on a gray-scale anatomic for immunocytochemistry, rat anti-Cyclin B1 (1:five hundred) (Santa Cruz Biotechnology) was utilised in addition to antibodies utilized in immunoblot evaluation demonstrated

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RealTime PCR reactions have been carried out in triplicate in microamp optical 384-properly plates...
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The effects ended up exhibited as a pseudocolor photon count graphic superimposed on a gray-scale anatomic for immunocytochemistry, rat anti-Cyclin B1 (a single:5 hundred) (Santa Cruz Biotechnology) was used in addition to antibodies utilized in immunoblot examination shown

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DMSO, PI, RNase A and Triton X-100 had been received from Sigma-Aldrich Corp. (St....
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The results ended up exhibited as a pseudocolor photon depend picture superimposed on a grey-scale anatomic for immunocytochemistry, rat anti-Cyclin B1 (1:500) (Santa Cruz Biotechnology) was used in addition to antibodies utilised in immunoblot analysis shown

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HEK293FT cells (Invitrogen) ended up transfected with the lentivirus constructs pCMV-VSV-G-RSV-Rev and pCAG-HIVgp by...