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January 19, 2017Updated January 19, 2017

Since fluorescence of a recycled receptor is quenched by the antiAlexa488 antibody at the cell surface, the reduction in total fluorescence represents a measure for recycling of the receptor

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Equivalent quantities of homogenates, postnuclear supernatant, microsomal fraction or purified PM have been operate...

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January 18, 2017Updated January 18, 2017

Short interfering RNA (siRNA) were designed and synthesized against exon 4 of the rat CARP sequence, rat GATA4 sequence

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Lifestyle medium contained Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with seven% fetal bovine...

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January 17, 2017Updated January 17, 2017

Similar to Angeli’s salt, the hypertrophic response to Ang II in neonatal rat cardiomyocytes is also prevented by the natriuretic peptide and particulate guanylyl cyclase

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Comparable to Angeli’s salt, the hypertrophic reaction to Ang II in neonatal rat cardiomyocytes...

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January 16, 2017Updated January 16, 2017

When injected s.c. into nude mice, CC4A gave rise to a high-grade SCC or spindle cell carcinoma (or SCC IV), whereas CC4B gave rise to a well- differentiated

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When injected s.c. into nude mice, CC4A gave increase to a substantial-quality SCC or...

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January 12, 2017Updated January 12, 2017

The signal was detected by probing with anti-Digoxigenin-AP (Roche), Fab fragments from a sheep anti-digoxigenin antibody conjugated with alkaline phosphatase

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H1299 cells have been managed in RPMI 1640 containing 10 mM HEPES with ten%...

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January 11, 2017Updated January 11, 2017

Recent reports use tyrosine kinase inhibitors for prevention of diabetes and diabetic complications

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Immunofluorescent staining of CD68 and LYVE-1 in pancreas from C57BL/six mice. Scale bars: a...

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January 10, 2017Updated January 10, 2017

To determine the transfection efficiency HEK 293T cells were simultaneously transfected with Green Fluorescent Protein

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The transfection mixture was remaining to incubate for two hundred min. at place temperature...

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January 9, 2017Updated January 9, 2017

Vascular endothelial cells in vivo are constantly exposed to ROS that are released from neutrophils, macrophages, and vascular smooth muscle cells

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Vascular endothelial cells in vivo are continuously exposed to ROS that are released from...

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January 6, 2017Updated January 6, 2017

Cells were resuspended in 500 mL of the buffer and fluorescence was measured using a FACSCalibur flow cytometer

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Pursuing incubation, the cells ended up washed in PBS and centrifuged at 8006g for...

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January 5, 2017Updated January 5, 2017

The samples were then classified into 4 groups based on disease category and ET patients were divided into JAK2 V617Fpositive

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We concentrated on SOCS3 and SPI1 in our subsequent research. Amid the fourteen genes...